| 1 | Kristin Culp | 12 | West Central Valley High School | 1:04.84 | | 3 |
| 2 | Annika Wall | 12 | Jesup High School | 1:05.50 | | 3 |
| 3 | Paige Miller | 12 | West Branch High School | 1:05.79 | | 3 |
| 4 | Hannah Wagner | 12 | Central Springs High School | 1:06.68 | | 3 |
| 5 | Anmarie Stuit | 11 | Unity Christian High School | 1:06.74 | | 2 |
| 6 | Jaycie Schueman | 12 | A-H-S-T High School | 1:07.44 | | 3 |
| 7 | Mikayla Turek | 10 | Unity Christian High School | 1:07.45 | | 2 |
| 8 | Ryin Lehmann | 10 | North Fayette High School | 1:07.87 | | 2 |
| 9 | Morgan Fopma | 10 | Pella Christian High School | 1:08.13 | | 3 |
| 10 | Isabelle Gradwell | 11 | West Marshall High School | 1:08.25 | | 2 |
| 11 | Lisa Tucker | 12 | Cascade, Western Dubuque High School | 1:08.43 | | 2 |
| 12 | Sarah Vorwerk | 12 | Mediapolis High School | 1:09.15 | | 3 |
| 13 | Olivia Laabs | 9 | Guthrie Center High School | 1:09.46 | | 1 |
| 14 | Tessa Fields | 11 | North Cedar High School | 1:09.47 | | 2 |
| 15 | Calli Wareham | 11 | OA-BCIG High School | 1:09.55 | | 1 |
| 16 | Shania Boyd | 12 | Columbus Community High School | 1:09.74 | | 1 |
| 17 | Jayci Vos | 12 | PCM High School | 1:10.28 | | 3 |
| 18 | Alicia Turnquist | 11 | Alta-Aurelia High School | 1:10.33 | | 1 |
| 19 | Jenna Willett | 12 | BCLUW High School | 1:10.36 | | 2 |
| 20 | Lynette Wuebker | 12 | Roland-Story High School | 1:10.65 | | 2 |
| 21 | Adrianna Holmes | 11 | Shenandoah High School | 1:11.19 | | 1 |
| 22 | Hailey Sanders | 9 | Louisa-Muscatine High School | 1:11.31 | | 1 |
| 23 | Helena Fantz | 12 | North Fayette High School | 1:11.40 | | 1 |
| 24 | Jillian Heitland | 11 | Garner-Hayfield High School | 1:12.32 | | 1 |
