| Athlete | 2025 | 2024 | Difference |
| Scarlett Dwyer - Cedar Rapids Washington | 2:41.09 | 3:22.00 | -40.91 |
| Genesis Reyes - Liberty | 2:56.36 | 3:35.00 | -38.64 |
| Caejyn Helgens - Anamosa | 2:49.60 | 3:21.20 | -31.6 |
| Ally Adams - Underwood High School | 2:32.83 | 3:00.47 | -27.64 |
| Bianca Seaton - South Hamilton High School | 2:37.16 | 3:02.43 | -25.27 |
| Amy Harbaugh - Dike-New Hartford High School | 3:06.33 | 3:31.20 | -24.87 |
| Elizabeth Moore - Martensdale-St. Marys High School | 2:40.47 | 3:04.73 | -24.26 |
| Chloe Bowling - Easton Valley | 3:06.99 | 3:30.94 | -23.95 |
| Marissa Eads - Muscatine | 2:49.85 | 3:11.20 | -21.35 |
| Caroline Rupe - Mount Vernon | 2:30.09 | 2:51.18 | -21.09 |
| Isabel Grossoehme - Webster City High School | 2:29.87 | 2:49.57 | -19.7 |
| Katelyn Wade - Central Decatur High School | 3:39.23 | 3:58.60 | -19.37 |
| Marissa Pewe - Johnston High School | 2:20.43 | 2:39.38 | -18.95 |
| Elyse Bierman - Indianola High School | 2:43.68 | 3:02.57 | -18.89 |
| Jayden Kluver - Le Mars High School | 2:50.18 | 3:08.89 | -18.71 |
| Ryleigh Thomas - Treynor High School | 2:53.59 | 3:10.76 | -17.17 |
| Addison Guerra - West Liberty | 2:46.14 | 3:03.29 | -17.15 |
| Sadie Alberti - Harlan High School | 2:34.81 | 2:51.58 | -16.77 |
| Callie Deutschle - Pekin High School | 2:45.51 | 3:02.11 | -16.6 |
| Sophia Hughes - Des Moines Hoover High School | 2:49.20 | 3:05.22 | -16.02 |
| Kaelea Blakely - Garner-Hayfield | 3:44.11 | 3:59.23 | -15.12 |
| Kienna Lassen - MOC-Floyd Valley High School | 2:24.61 | 2:39.26 | -14.65 |
| Lydia Georgick - A-D-M High School | 2:44.75 | 2:58.16 | -13.41 |
| Abbi Waskow - Dunkerton | 2:22.70 | 2:35.83 | -13.13 |
| Kalli Titus - Iowa City West | 2:45.59 | 2:58.64 | -13.05 |
| Jenny Claussen - Central DeWitt | 2:34.95 | 2:47.81 | -12.86 |
| Haylee Hughes - Glenwood High School | 2:35.50 | 2:48.28 | -12.78 |
| Hope Henderson - Creston High School | 2:30.48 | 2:43.15 | -12.67 |
| Lise Hoyer - Dubuque Senior | 2:29.20 | 2:41.77 | -12.57 |
| Lizeth Esqueda - Cedar Rapids Jefferson | 2:44.40 | 2:56.80 | -12.4 |
| Rebekah Steinbron - Clear Lake | 2:28.90 | 2:40.85 | -11.95 |
| Anna Galvin - Denison-Schleswig High School | 3:25.36 | 3:37.15 | -11.79 |
| Lucy Lueken - Dubuque Wahlert | 2:32.94 | 2:44.45 | -11.51 |
| Grace Anton - East Buchanan | 3:18.60 | 3:30.07 | -11.47 |
| Kenzie Douglas - Lenox High School | 3:04.01 | 3:15.46 | -11.45 |
| Caroline Krug - Ames High School | 2:34.75 | 2:46.15 | -11.4 |
| Ella Boswell - Orient-Macksburg High School | 2:58.21 | 3:09.54 | -11.33 |
| Cassie Swisher - West Branch | 3:04.62 | 3:15.87 | -11.25 |
| Lanie Hanna - Saint Ansgar High School | 2:37.82 | 2:49.00 | -11.18 |
| Jillian Koenig - Davenport North | 2:53.74 | 3:04.83 | -11.09 |
| Emalee Moorman - Wayne High School | 2:56.09 | 3:06.94 | -10.85 |
| Lily Myers - Perry High School | 2:29.92 | 2:40.22 | -10.3 |
| Brynn Kartman - West Delaware | 2:24.80 | 2:35.00 | -10.2 |
| Lily Young - Lake Mills High School | 2:53.69 | 3:03.59 | -9.9 |
| Reese Wiley - Prairie | 2:20.56 | 2:30.42 | -9.86 |
| Breyer Anderson - Alta-Aurelia High School | 2:32.01 | 2:41.76 | -9.75 |
| Madeline Knispel - Treynor High School | 2:30.23 | 2:39.89 | -9.66 |
| Emee Dani - Waukee Northwest | 2:15.44 | 2:24.83 | -9.39 |
| Jr Chaney Lyndsey - CAM High School | 2:52.08 | 3:01.40 | -9.32 |
| Sophia Campbell - North Polk High School | 2:45.05 | 2:54.26 | -9.21 |
| Sydney Hull - East Marshall High School | 2:30.96 | 2:40.14 | -9.18 |
| Gracie Uhl - Marion | 4:04.37 | 4:13.54 | -9.17 |
| Marcella Palmer - Sioux City North High School | 2:40.23 | 2:49.28 | -9.05 |
| Karina Bloom - Sioux Central High School | 2:28.66 | 2:37.40 | -8.74 |
| Allison Sheriff - Waukee Northwest | 2:18.83 | 2:27.49 | -8.66 |
| Hannah Brandhorst - Madrid High School | 2:28.85 | 2:37.47 | -8.62 |
| Evelyn Moeller - Mount Vernon | 2:14.09 | 2:22.52 | -8.43 |
| Peyton Ray - Newton High School | 2:40.60 | 2:49.02 | -8.42 |
| Bry Morgan - Boone High School | 2:40.21 | 2:48.54 | -8.33 |
| Gracie Krumm - Waukee | 2:40.21 | 2:48.51 | -8.3 |
| Ava Paulsen - A-H-S-T High School | 2:31.30 | 2:39.48 | -8.18 |
| Lizzy McCann - Van Meter High School | 2:32.64 | 2:40.79 | -8.15 |
| Alaina Gourley - Danville | 2:20.31 | 2:28.43 | -8.12 |
| Josee Simonson - Cedar Falls | 2:15.38 | 2:23.45 | -8.07 |
| Addison Doolittle - Mount Ayr High School | 2:39.74 | 2:47.79 | -8.05 |
| Hannah Kissel - Louisa-Muscatine | 2:52.88 | 3:00.91 | -8.03 |
| Payton Davis - Creston High School | 2:39.04 | 2:47.02 | -7.98 |
| Ashlyn Hoffman - Williamsburg | 2:35.63 | 2:43.54 | -7.91 |
| Raya Miller - Ballard High School | 2:33.96 | 2:41.75 | -7.79 |
| Olivia Halfpap - Van Meter High School | 2:27.16 | 2:34.90 | -7.74 |
| Molly Isakson - Sioux Center High School | 2:21.25 | 2:28.94 | -7.69 |
| Dylan Moeller - Davenport Central | 2:20.15 | 2:27.78 | -7.63 |
| Kennedy Kolbet - West Delaware | 2:32.56 | 2:40.18 | -7.62 |
| Grace Steinmetz - Heartland Christian School | 2:33.83 | 2:41.37 | -7.54 |
| Sydney Dee - Solon | 2:29.07 | 2:36.55 | -7.48 |
| Aubrey Renander - Clarinda High School | 3:10.86 | 3:18.32 | -7.46 |
| Serene THOMPSON - Albia High School | 2:35.84 | 2:43.29 | -7.45 |
| Leah Kincaid - Ames High School | 2:29.60 | 2:36.92 | -7.32 |
| Gillian Schramm - Ankeny High School | 2:26.81 | 2:34.05 | -7.24 |
| Eastin Whisner - Mount Vernon | 2:25.01 | 2:32.17 | -7.16 |
| Makenna Kurth - Waukon | 2:50.63 | 2:57.77 | -7.14 |
| Laicey Lutz - Panorama High School | 2:41.08 | 2:48.00 | -6.92 |
| Anne Johnson - Waukee | 2:38.03 | 2:44.92 | -6.89 |
| Ava Hoover - North Linn | 2:44.64 | 2:51.42 | -6.78 |
| Cameron Shumpert - Davenport Central | 2:41.02 | 2:47.67 | -6.65 |
| Jada Hoffman - Assumption | 2:23.08 | 2:29.66 | -6.58 |
| Brooklyn Heesch - Clear Lake | 2:45.93 | 2:52.48 | -6.55 |
| Katie Rush - Waukee Northwest | 2:19.63 | 2:26.05 | -6.42 |
| Cheyenne Dike - East Union High School | 3:01.12 | 3:07.52 | -6.4 |
| Ella HOWELL - Louisa-Muscatine | 2:41.89 | 2:48.27 | -6.38 |
| Emma Reif - North Scott | 2:42.88 | 2:49.17 | -6.29 |
| Olivia Fehn - Johnston High School | 2:12.98 | 2:19.24 | -6.26 |
| Anika Roe - Prairie | 2:35.41 | 2:41.66 | -6.25 |
| Madelyn Grothus - Madrid High School | 2:34.93 | 2:41.07 | -6.14 |
| Claire Weaver - Forest City High School | 2:30.00 | 2:36.13 | -6.13 |
| Elyse Zwart - Boyden-Hull High School | 2:25.83 | 2:31.94 | -6.11 |
| Emma Sherman - Des Moines Roosevelt High School | 2:27.08 | 2:33.05 | -5.97 |
| Ellie Anton - Cedar Falls | 2:58.71 | 3:04.62 | -5.91 |
| Ava Brummer - Tipton | 2:38.48 | 2:44.34 | -5.86 |
| Natalie Anderson - MOC-Floyd Valley High School | 2:23.30 | 2:29.07 | -5.77 |
| Isabella Stika - Turkey Valley | 2:30.95 | 2:36.71 | -5.76 |
| Grace DeRocher - Norwalk High School | 2:34.19 | 2:39.90 | -5.71 |
| Sophia Graber - Ankeny High School | 2:25.54 | 2:31.20 | -5.66 |
| Sarah Tilberg - Le Mars High School | 2:54.75 | 3:00.37 | -5.62 |
| Layla Shipley - Solon | 2:45.96 | 2:51.42 | -5.46 |
| Hannah Haaland - North Polk High School | 2:27.97 | 2:33.41 | -5.44 |
| Ella Pitz - Sumner-Fredericksburg High School | 2:44.12 | 2:49.44 | -5.32 |
| Maria Hart - Dubuque Hempstead | 2:50.89 | 2:56.19 | -5.3 |
| Fr Autumn Harter - Bondurant-Farrar High School | 2:42.62 | 2:47.84 | -5.22 |
| Lamise Ajram - Prairie | 2:35.96 | 2:41.16 | -5.2 |
| Kylie Olson - Marion | 3:06.58 | 3:11.78 | -5.2 |
| Sarah May - MOC-Floyd Valley High School | 2:28.20 | 2:33.39 | -5.19 |
| Claire Locher - Iowa City | 2:34.02 | 2:39.16 | -5.14 |
| Addison Golinghorst - Davenport West | 2:38.79 | 2:43.71 | -4.92 |
| Marissa Ferebee - Pella High School | 2:25.64 | 2:30.42 | -4.78 |
| Ashlynn Salter - Sergeant Bluff-Luton Gigh School | 2:53.72 | 2:58.40 | -4.68 |
| Megan Gardiner - Sioux City North High School | 2:48.24 | 2:52.87 | -4.63 |
| Katie Campbell - Ames High School | 2:30.07 | 2:34.62 | -4.55 |
| Lizzie Neumann - Pella High School | 2:24.97 | 2:29.38 | -4.41 |
| Marlee Maas - Hampton-Dumont High School | 3:03.05 | 3:07.46 | -4.41 |
| Layla Strode - Centerville High School | 2:53.29 | 2:57.69 | -4.4 |
| AItchison Sophie - West Hancock High School | 2:46.94 | 2:51.32 | -4.38 |
| Kate Van Waning - Iowa City West | 2:22.43 | 2:26.72 | -4.29 |
| Jayna Lamping - Johnston High School | 2:24.60 | 2:28.79 | -4.19 |
| Lilli Green - Western Dubuque | 3:32.47 | 3:36.66 | -4.19 |
| Madeline Paustian - Davenport West | 2:45.60 | 2:49.76 | -4.16 |
| Kayley Snowgren - Van Buren High School | 2:29.44 | 2:33.49 | -4.05 |
| Bradi Houston - Davis County High School | 2:37.15 | 2:41.12 | -3.97 |
| Alyvia Anderson - Washington High School | 2:50.09 | 2:54.04 | -3.95 |
| Aubrielle Tousey - Johnston High School | 2:31.28 | 2:35.14 | -3.86 |
| Tori Koenigs - Riceville High School | 2:54.39 | 2:58.21 | -3.82 |
| Kasey McIlrath - Western Dubuque | 2:44.33 | 2:48.05 | -3.72 |
| Ashlynn Sauser - Oelwein High School | 2:29.69 | 2:33.40 | -3.71 |
| Jordyn Brown - Indianola High School | 2:23.11 | 2:26.81 | -3.7 |
| Chloe Rausch - Prairie | 2:57.32 | 3:00.99 | -3.67 |
| Sedona Oldenburger - Dike-New Hartford High School | 2:28.76 | 2:32.40 | -3.64 |
| Olivia Lindeman - Linn-Mar | 2:44.65 | 2:48.27 | -3.62 |
| Julia Mortvedt - Ames High School | 2:42.86 | 2:46.47 | -3.61 |
| Madison Hoffert - Mount Vernon | 2:30.19 | 2:33.73 | -3.54 |
| Kadence Huck - Nashua-Plainfield High School | 2:10.51 | 2:13.98 | -3.47 |
| Summer Kurth - West Marshall High School | 2:51.69 | 2:55.13 | -3.44 |
| Mila Grothus - Madrid High School | 2:46.79 | 2:50.22 | -3.43 |
| Ava Van Ee - Dowling Catholic High School | 2:33.36 | 2:36.75 | -3.39 |
| Dalanie Graap - Lone Tree High School | 3:11.84 | 3:15.21 | -3.37 |
| Raegan Wyant - Clear Creek-Amana | 2:36.52 | 2:39.87 | -3.35 |
| Bethany Guertin - Bondurant-Farrar High School | 2:38.30 | 2:41.61 | -3.31 |
| Abi Teeter - PCM High School | 2:32.51 | 2:35.75 | -3.24 |
| Emma Truesdell - Roland-Story High School | 2:41.19 | 2:44.43 | -3.24 |
| Finley Hancock - Valley High School | 2:25.29 | 2:28.47 | -3.18 |
| Addison Bertrand - Bishop Heelan Catholic High School | 2:26.51 | 2:29.63 | -3.12 |
| Delayna Reese - Lewis Central High School | 2:24.60 | 2:27.70 | -3.1 |
| Kora McCollough - Vinton-Shellsburg High School | 3:09.19 | 3:12.29 | -3.1 |
| Alexa Williams - A-D-M High School | 2:45.16 | 2:48.21 | -3.05 |
| Heather Mendenhall - Southwest Valley High School | 3:13.02 | 3:16.06 | -3.04 |
| Halle Olson - Dallas Center-Grimes High School | 2:52.83 | 2:55.71 | -2.88 |
| Elle Harms - West Fork High School | 2:52.98 | 2:55.80 | -2.82 |
| Kyrin DeGroot - Western Christian High School | 2:34.84 | 2:37.62 | -2.78 |
| Bailey Wheeler - PCM High School | 2:40.40 | 2:43.13 | -2.73 |
| Isabell Hodgson - Hinton High School | 2:53.04 | 2:55.73 | -2.69 |
| Lilly Graham - Dubuque Wahlert | 2:42.61 | 2:45.24 | -2.63 |
| Makenna Madetzke - Ankeny High School | 2:14.03 | 2:16.65 | -2.62 |
| Jana Harms - West Fork High School | 3:04.08 | 3:06.68 | -2.6 |
| Amberley Gerholdt - Nashua-Plainfield High School | 3:06.17 | 3:08.75 | -2.58 |
| Olivia Beaune - South Hamilton High School | 2:40.02 | 2:42.59 | -2.57 |
| Lila Swenson - Belmond-Klemme High School | 2:39.44 | 2:41.87 | -2.43 |
| Corinne McCord - Sioux Center High School | 2:34.82 | 2:37.24 | -2.42 |
| Fr Ashley Wong - Dallas Center-Grimes High School | 3:04.71 | 3:07.13 | -2.42 |
| Harper Souhrada - Johnston High School | 2:37.62 | 2:40.01 | -2.39 |
| Kate Baker - Indianola High School | 3:00.91 | 3:03.23 | -2.32 |
| Hannah Higgins - Clarinda High School | 2:54.69 | 2:56.74 | -2.05 |
| Clare Haselhoff - Bondurant-Farrar High School | 3:11.12 | 3:13.14 | -2.02 |
| Alexis Cummins - North Union High School | 2:33.57 | 2:35.55 | -1.98 |
| Bethany Warren - Forest City High School | 2:27.56 | 2:29.51 | -1.95 |
| Kierstyn Cameron - Kingsley-Pierson High School | 2:54.00 | 2:55.86 | -1.86 |
| Greta Vos - Pella Christian High School | 2:39.84 | 2:41.55 | -1.71 |
| Ava Freund - Harlan High School | 2:47.18 | 2:48.89 | -1.71 |
| Lauren Valleroy - Davenport Central | 3:10.01 | 3:11.69 | -1.68 |
| Fr Brooklyn Schafer - Adair-Casey/Guthrie Center | 2:43.81 | 2:45.42 | -1.61 |
| Ava England - Mount Ayr High School | 2:53.58 | 2:55.19 | -1.61 |
| Addy Henkelvig - Danville | 2:44.45 | 2:46.04 | -1.59 |
| Ava Meinders - North Iowa High School | 2:35.96 | 2:37.53 | -1.57 |
| Keira Andersen - Gilbert High School | 2:19.66 | 2:21.19 | -1.53 |
| Khloee Weitzel - Sioux City East High School | 2:46.90 | 2:48.41 | -1.51 |
| Ava Vanderheyden - Nevada High School | 2:42.21 | 2:43.71 | -1.5 |
| Sloane Moeller - Davenport Central | 2:28.55 | 2:30.04 | -1.49 |
| Kaelin Carlson-Gross - North Scott | 2:45.00 | 2:46.49 | -1.49 |
| Edie Axmear - Williamsburg | 2:30.14 | 2:31.61 | -1.47 |
| Nora Ford - Alburnett | 2:53.28 | 2:54.74 | -1.46 |
| Fr Maven Smith - Winterset High School | 2:33.44 | 2:34.72 | -1.28 |
| Lillian Knapp - Interstate 35 High School | 2:41.83 | 2:43.11 | -1.28 |
| Shyanne Nicklaus - Janesville High School | 3:12.25 | 3:13.42 | -1.17 |
| Victoria Stellmach - Bettendorf | 2:38.88 | 2:40.00 | -1.12 |
| Sarah Feddersen - Gilbert High School | 2:14.80 | 2:15.88 | -1.08 |
| Gabby Hingtgen - Bellevue | 2:40.92 | 2:41.98 | -1.06 |
| Emma Parkinson - Lynnville-Sully High School | 2:43.62 | 2:44.67 | -1.05 |
| Hannah Friedlein - Central | 3:50.87 | 3:51.91 | -1.04 |
| Falyn Svalstad - Solon | 2:27.63 | 2:28.57 | -0.94 |
| Reaghan Ressler - Independence High School | 2:26.96 | 2:27.84 | -0.88 |
| Klaire Lietz - Valley High School | 2:20.92 | 2:21.78 | -0.86 |
| Addison Dooley - Dallas Center-Grimes High School | 2:27.12 | 2:27.96 | -0.84 |
| Harper Erwin - Regina | 2:40.38 | 2:41.15 | -0.77 |
| Gwen Tichy - Baxter High School | 2:50.82 | 2:51.41 | -0.59 |
| Greta Hansen - Marion | 2:24.26 | 2:24.80 | -0.54 |
| Emma Stanley - Ames High School | 2:28.07 | 2:28.54 | -0.47 |
| Lili Denton - St. Albert High School | 2:14.83 | 2:15.27 | -0.44 |
| Autumn Newman - Glenwood High School | 3:05.35 | 3:05.68 | -0.33 |
| Cambell Crabb - Southeast Polk High School | 3:14.10 | 3:14.34 | -0.24 |
| Khloe Parker - East Union High School | 3:06.93 | 3:07.16 | -0.23 |
| Alaina Safly - Mid-Prairie | 3:08.00 | 3:08.19 | -0.19 |
| Leya Walker - Red Oak High School | 3:22.39 | 3:22.58 | -0.19 |
| Grace Storjohann - Grundy Center High School | 2:15.63 | 2:15.78 | -0.15 |
| Ava Huffman - North Mahaska High School | 2:42.20 | 2:42.26 | -0.06 |
| Elise Huegerich - Dowling Catholic High School | 2:27.47 | 2:27.51 | -0.04 |